Electrophoretic Study of Saliva †

نویسندگان

  • Thorn Kinersly
  • H. B. Leite
چکیده

Human saliva is an easily obtained, abundant secretion, but the native proteins in it seem to have been little studied. Only salivary amylase has been isolated in a pure state. In regard to electrophoresis, the phase boundary apparatus has been used by Blix' to study animal submaxillary mucin, by Bernfeld, Staub, and Fischer1 and Muus to test the homogeneity of purified salivary amylase, and by Mack, Wolf, and Stern' to investigate salivary proteins in gastric juice. Paper electrophoretic separation of protein elements in whole saliva was previously reported by one of us.7 In this study, paper electrophoresis was used to investigate concentrated and nonconcentrated saliva with and without the in vitro addition of bromphenol blue, calcium,' and iron.' The migration of the salivary blood group specific substances A and B was also studied.

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عنوان ژورنال:
  • The Yale Journal of Biology and Medicine

دوره 29  شماره 

صفحات  -

تاریخ انتشار 1957